Types of Water

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• Distilled water: water that has been evaporated and condensed to increase purity
• Deionized (DI) water: water that has ions removed using ion exchange (e..g., osmosis)
• MilliQ water: is DI water but with higher purity. Water has been deionised/demineralised and gone through filtration. MilliQ water can replace DI water but not the other way around. This is usually used for autovclave, water baths, buffers, etc
• Nuclease free water- Distilled water free of DNAse and RNAse. used for PCR, sequencing, and buffers
• Molecular grade water: Ultrapure water (basically a combination of Milli-Q and Nuclease free). Used for sensitice processes e.g., PCR, qPCR, RT-PCR, etc